Role for YakA, cAMP, and protein kinase A in regulation of stress responses of Dictyostelium discoideum cells.

ABSTRACT: The Dictyostelium protein kinase YakA is required for the growth-to-development transition. During growth YakA controls the cell cycle, regulating the intervals between cell divisions. When starved for nutrients Dictyostelium cells arrest growth and undergo changes in gene expression, decreasing vegetative mRNAs and inducing the expression of pkaC. YakA is an effector of these changes, being necessary for the decrease of vegetative mRNA expression and the increase of protein kinase A (PKA) activity that will ultimately regulate expression of adenylyl cyclase, cAMP synthesis, and the induction of development. We report a role for this kinase in the response to nitrosoative or oxidative stress of Dictyostelium cells. Hydrogen peroxide and sodium nitroprusside arrest the growth of cells and t

SUBMITTER: Taminato A 

PROVIDER: S-EPMC117311 | biostudies-literature | 2002 Jul

REPOSITORIES: biostudies-literature