Molecular subtyping of Blastocystis sp. detected in patients at a large tertiary referral hospital in Lusaka, Zambia.

ABSTRACT:

Background

Blastocystis sp. is a common enteric eukaryote of humans whose pathogenicity is still debatable. However, a number of reported Blastocystis colonization associated with enteric disease exist. In Zambia, presence of the pathogen has previously been reported in children. However, the molecular epidemiology of Blastocystis colonization remains unclarified in Zambia.

Methods and results

Archived stool samples submitted for routine parasitological diagnosis at Zambia's largest tertiary referral hospital positive for Blastocystis sp. by microscopic examination were selected for the study. Subtyping of the Blastocystis was done based on polymerase chain reactions (PCR) amplification, sequencing and subsequent phylogenetic analysis of th

SUBMITTER: Munsaka G 

PROVIDER: S-EPMC11731786 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature