Optimizing the xWORM assay for monitoring hookworm larvae motility.

ABSTRACT: Parasitic worms (helminths) infect almost all taxa across the animal kingdom, and pose significant challenges to public health and economies, particularly in developing countries. To address this problem, researchers have developed various tools to measure the motility and viability of helminths. However, the conditions used in anthelmintic screening assays are often not optimized, and can vary considerably between research teams. These unoptimized conditions may impact novel drug screens, as little is known about the effects of different conditions on the health of the target parasites. To improve future research, this study determined the effects of key assay parameters including, media type, media concentration, in-well parasite density, and assay duration on the infective third-stage l

SUBMITTER: Lennox-Bulow D 

PROVIDER: S-EPMC11731822 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature