The fly route of extended-spectrum-β-lactamase-producing Enterobacteriaceae dissemination in a cattle farm: from the ecosystem to the molecular scale.

ABSTRACT:

Introduction

This study aimed to understand the origin and to explain the maintenance of extended-spectrum β-lactamase (ESBL) Enterobacteriaceae isolated from food-producing animals in a third-generation cephalosporin (3GC)-free farm.

Methods

Culture and molecular approaches were used to test molecules other than 3GC such as antibiotics (tetracycline and oxytetracycline), antiparasitics (ivermectin, flumethrin, fenbendazol, and amitraz), heavy metal [arsenic, HNO₃, aluminum, HNO₃, cadmium (CdSO₄), zinc (ZnCl₂), copper (CuSO₄), iron (FeCl₃), and aluminum (Al₂SO₄)], and antioxidant (butylated hydroxytoluene) as sources of selective pressure. Whole-genome sequencing using short read (

SUBMITTER: Caderhoussin A 

PROVIDER: S-EPMC11732033 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature