Manipulation of mitochondrial poly(A) polymerase family proteins in Trypanosoma brucei impacts mRNA termini processing.

ABSTRACT: RNA-specific nucleotidyltransferases (rNTrs) add nontemplated nucleotides to the 3^' end of RNA. Two noncanonical rNTRs that are thought to be poly(A) polymerases (PAPs) have been identified in the mitochondria of trypanosomes - KPAP1 and KPAP2. KPAP1 is the primary polymerase that adds adenines (As) to trypanosome mitochondrial mRNA 3^' tails, while KPAP2 is a non-essential putative polymerase whose role in the mitochondria is ambiguous. Here, we elucidate the effects of manipulations of KPAP1 and KPAP2 on the 5^' and 3^' termini of transcripts and their 3^' tails. Using glycerol gradients followed by immunoblotting, we present evidence that KPAP2 is found in protein complexes of up to about 1600 kDa. High-throughput sequencing of mRNA ter

SUBMITTER: Smoniewski CM 

PROVIDER: S-EPMC11732105 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature