Identification of biomarker candidates for filarial parasite infections by analysis of extracellular vesicles.

ABSTRACT:

Background

Improved diagnostic tools are needed for detecting active filarial infections in humans. Tests are available that detect adult W. bancrofti circulating filarial antigen, but there are no sensitive and specific biomarker tests for brugian filariasis or loiasis. Here we explored whether extracellular vesicles released by filarial parasites contain diagnostic biomarker candidates.

Methods

Vesicles were isolated using VN96-affinity purification from supernatants of short-term in vitro cultured B. malayi microfilariae (Mf) and analyzed by mass spectrometry (Bruker timsTOF). Parasite-specific proteins were identified by bioinformatic analysis and a protein was called present if supported by ≥ 2 spectra. After validation with cultures parasites, this

SUBMITTER: Yates D 

PROVIDER: S-EPMC11732158 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature