Exercise-induced methylation of the Serhl2 promoter and implication for lipid metabolism in rat skeletal muscle.

ABSTRACT:

Objectives

Environmental factors such as physical activity induce epigenetic modifications, with exercise-responsive DNA methylation changes occurring in skeletal muscle. To determine the skeletal muscle DNA methylation signature of endurance swim training, we used whole-genome methylated DNA immunoprecipitation (MeDIP) sequencing.

Methods

We utilized endurance-trained rats, cultured L6 myotubes, and human skeletal muscle cells, employing MeDIP sequencing, gene silencing, and palmitate oxidation assays. Additional methods included promoter luciferase assays, fluorescence microscopy, and RNA/DNA analysis to investigate exercise-induced molecular changes.

Results

Gene set enrichment analysis (GSEA) of differentially methylated promoter regions identified an enrichment o

SUBMITTER: Katayama M 

PROVIDER: S-EPMC11732562 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature