Petrous bones versus tooth cementum for genetic analysis of aged skeletal remains.

ABSTRACT: A proper sampling strategy is important to obtain sufficient DNA for successful identification of aged skeletal remains. The petrous bone is the highest DNA-yielding bone in the human body. Because DNA extraction from the petrous bone is very destructive, the demand for other DNA sources is significant. When investigating aged skeletal remains, teeth are usually preserved, and recent studies have shown that DNA in teeth can be best preserved in the dental cementum that surrounds the surface of the tooth root. To extract DNA from the surface of the tooth root, a nondestructive method without grinding was used. Petrous bones and teeth from 60 archaeological adult skeletons were analyzed. The DNA yield, degree of DNA degradation, and STR typing success were compared, and the results showed hi

SUBMITTER: Zupanic Pajnic I 

PROVIDER: S-EPMC11732775 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature