Cytokine expression of soft tissue cells cultured with titanium discs and their respective supernatants in vitro.

ABSTRACT:

Objective

Titanium surface modifications improve osseointegration in dental and orthopedic implants. However, soft tissue cells can also reach the implant surface in immediate loading protocols. While previous research focused on osteogenic cells, the early response of soft tissue cells still needs to be better understood.

Material and methods

We have established a bioassay to this aim where human gingival fibroblasts, HSC2 oral squamous carcinoma cells, and murine bone marrow cells were cultured onto titanium discs or exposed to the respective supernatants for overnight. Modifications were double acid-etching (SLA), and coating with simulated body fluid (SBF) with or without odanacatib (ODN), a selective cathepsin K inhibitor reducing bone resorption.

Results

Our fin

SUBMITTER: Dos Santos Sanches N 

PROVIDER: S-EPMC11732886 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature