Addressing genome scale design tradeoffs in Pseudomonas putida for bioconversion of an aromatic carbon source.

ABSTRACT: Genome-scale metabolic models (GSMM) are commonly used to identify gene deletion sets that result in growth coupling and pairing product formation with substrate utilization and can improve strain performance beyond levels typically accessible using traditional strain engineering approaches. However, sustainable feedstocks pose a challenge due to incomplete high-resolution metabolic data for non-canonical carbon sources required to curate GSMM and identify implementable designs. Here we address a four-gene deletion design in the Pseudomonas putida KT2440 strain for the lignin-derived non-sugar carbon source, p-coumarate (p-CA), that proved challenging to implement. We examine the performance of the fully implemented design for p-coumarate to glutamine, a useful biomanufacturing intermediat

SUBMITTER: Banerjee D 

PROVIDER: S-EPMC11732973 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature