Building a reliable 16S mini-barcode library of wild bees from Occitania, south-west of France.

ABSTRACT:

Background

DNA barcoding and metabarcoding are now powerful tools for studying biodiversity and especially the accurate identification of large sample collections belonging to diverse taxonomic groups. Their success depends largely on the taxonomic resolution of the DNA sequences used as barcodes and on the reliability of the reference databases. For wild bees, the barcode sequences coverage is consistently growing in volume, but some incorrect species annotations need to be cared for. The COI (Cytochrome Oxydase subunit 1) gene, the most used in barcoding/metabarcoding of arthropods, suffers from primer bias and difficulties for covering all wild bee species using the classical Folmer primers.

New information

We present here a curated database for a 250 bp mini-barcode regi

SUBMITTER: Marquisseau A 

PROVIDER: S-EPMC11733625 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature