Promoter engineering with programmable upstream activating sequences in Aspergillus Niger cell factory.

ABSTRACT:

Background

Aspergillus niger is an important industrial filamentous fungus used to produce organic acids and enzymes. A wide dynamic range of promoters, particularly strong promoters, are required for fine-tuning the regulation of gene expression to balance metabolic flux and achieve the high yields of desired products. However, the limited understanding of promoter architectures and activities restricts the efficient transcription regulation of targets in strain engineering in A. niger.

Results

In this study, we identified two functional upstream activation sequences (UAS) located upstream of the core promoters of highly expressed genes in A. niger. We constructed and characterized a synthetic promoter library by fusing the efficient UAS elements upstream of the strong cons

SUBMITTER: Zheng X 

PROVIDER: S-EPMC11734539 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature