Stationary phase effects in hydrophilic interaction liquid chromatographic separation of oligonucleotides.

ABSTRACT: The use of liquid chromatography coupled with mass spectrometry (LC-MS) for the characterization of oligonucleotides and nucleic acids is a powerful analytical method. Recently, hydrophilic interaction chromatography (HILIC) has been proposed as a reasonable alternative to ion-pair reversed phase separations of oligonucleotides prior to MS. A wide variety of HILIC stationary phase surface chemistries are currently available. Although their selectivity can be considerably different, few studies have compared these chemistries for LC-MS analysis of oligonucleotides. We evaluated ten different HILIC column chemistries to understand their capabilities for separating a variety of oligonucleotides. In general, we found that most columns were ineffective at separating larger (n > 15-mer) o

SUBMITTER: Abernathy S 

PROVIDER: S-EPMC11734610 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature