Retrospective discrimination of PNES and epileptic seizure types using blood RNA signatures.

ABSTRACT:

Objectives

The ability to differentiate epileptic- and non-epileptic events is challenging due to a lack of reliable molecular seizure biomarker that provide a retrospective diagnosis. Here, we use next generation sequencing methods on whole blood samples to identify changes in RNA expression following seizures.

Methods

Blood samples were obtained from 32 patients undergoing video electroencephalogram (vEEG) monitoring. Blood samples were collected in PaxGene tubes at baseline (admission) and following a seizure event (4-6 h and 24 h later or discharge). EEG and video of clinical events were reviewed by the clinical team and study epileptologist and were classified as epileptic seizure, psychogenic nonepileptic spell (PNES), or other. RNA was extracted from blood and RNA exp

SUBMITTER: Bullinger K 

PROVIDER: S-EPMC11735489 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature