Crosstalk between genomic variants and DNA methylation in FLT3 mutant acute myeloid leukemia.

ABSTRACT: Acute myeloid leukemia (AML) is a type of blood cancer with diverse genetic variations and DNA methylation alterations. By studying the interaction of gene mutations, expression, and DNA methylation, we aimed to gain valuable insights into the processes that lead to block differentiation in AML. We analyzed TCGA-LAML data (173 samples) with RNA sequencing and DNA methylation arrays, comparing FLT3 mutant (48) and wild-type (125) cases. We conducted differential gene expression analysis using cBioPortal, identified DNA methylation differences with ChAMP tool, and correlated them with gene expression changes. Gene set enrichment analysis (g:Profiler) revealed significant biological processes and pathways. ShinyGo and GeneCards were used to find potential transcription factors and their bindi

SUBMITTER: Dao B 

PROVIDER: S-EPMC11735749 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature