Graphite-Based Bio-Mimetic Nanopores for Protein Sequencing and Beyond.

ABSTRACT: Protein sequencing using nanopores represents the next frontier in bio-analytics. However, linearizing unfolded proteins and controlling their translocation speed through solid-state nanopores pose significant challenges in protein sequencing. In order to address these issues, this work proposes a biomimetic graphite-based nanopore construction. These nanopores feature a nanometer-sized pore with a constriction zone, mimicking the structure of the α-hemolysin protein pore. Our all-atom Molecular Dynamics simulations demonstrate the high practical potential of these nanopores by revealing how their charge state renders them complete ion-selective and generates an electro-osmotic flow. This study shows that this nanopore construction can detect peptides at the single amino acid level by anal

SUBMITTER: Das CK 

PROVIDER: S-EPMC11735877 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature