Protocol for isolating antigen-specific monoclonal antibodies from immunized mice utilizing the Beacon platform.

ABSTRACT: Isolation of antigen-specific plasma B cells had been challenging until the recent arrival of the Beacon platform. Leveraging light-sorting technology, Beacon can perform high-throughput screening of plasma B cells on a chip to sort single cells with the desired antigen specificity. Here, we present a protocol for isolating antigen-specific plasma B cells from immunized mice using Beacon, sequencing the encoded B cell receptors (BCRs), and cloning and expressing the resulting antibodies. This protocol can easily be extended to human samples.

SUBMITTER: Fu M 

PROVIDER: S-EPMC11735987 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature