Structural and functional analysis of a bile salt hydrolase from the bison microbiome.

ABSTRACT: The bile salt hydrolases (BSHs) are significant constituents of animal microbiomes. An evolving appreciation of their roles in health and disease has established them as targets of pharmacological inhibition. These bacterial enzymes belong to the N-terminal nucleophile superfamily and are best known to catalyze the deconjugation of glycine or taurine from bile salts to release bile acid substrates for transformation and or metabolism in the gastrointestinal tract. Here, we identify and describe the BSH from a common member of the Plains bison microbiome, Arthrobacter citreus (BSH_Ac). Steady-state kinetic analyses demonstrated that BSH_Ac is a broad-spectrum hydrolase with a preference for glycine-conjugates and deoxycholic acid (DCA). Second-order rate constants (k

SUBMITTER: Asar R 

PROVIDER: S-EPMC11736000 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature