Incorporation of recombinant proteins into extracellular vesicles by Lactococcus cremoris.

ABSTRACT: Extracellular vesicles (EVs) are nanosized lipid bilayer particles released by various cellular organisms that carry an array of bioactive molecules. EVs have diagnostic potential, as they play a role in intercellular interspecies communication, and could be applied in drug delivery. In contrast to mammalian cell-derived EVs, the study of EVs from bacteria, particularly Gram-positive bacteria, received less research attention. This study aimed to investigate the production of EVs by lactic acid bacterium Lactococcus cremoris NZ9000 and to examine the impact of recombinant protein expression on their formation and protein content. Four different recombinant proteins were expressed in L. cremoris NZ9000, in different forms of expression and combinations, and the produced EVs were isolated us

SUBMITTER: Plavec TV 

PROVIDER: S-EPMC11736121 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature