Single-cell landscape of alternative polyadenylation in human lymphoid hematopoiesis.

ABSTRACT: Alternative polyadenylation (APA) is an essential post-transcriptional process that produces mature mRNA isoforms by regulating the usage of polyadenylation sites (PASs). APA is involved in lymphocyte activation; however, its role throughout the entire differentiation trajectory remains elusive. Here, we analyzed single-cell 3'-end transcriptome data from healthy subjects to construct a dynamic-APA landscape from hematopoietic stem and progenitor cells (HSPCs) to terminally differentiated lymphocytes. This analysis covered 19973 cells of 12 clusters from five lineages (B cells, CD4+ T cells, CD8+ T cells, natural killer cells, and plasmacytoid dendritic cells). A total of 2364 genes exhibited differential 3'-untranslated region (3'UTR) PAS usage, and 3021 genes displayed differential intro

SUBMITTER: Qiang J 

PROVIDER: S-EPMC11736434 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature