Enhancer identification and activity evaluation in the red flour beetle, Tribolium castaneum.

ABSTRACT: Evolution of cis-regulatory elements (such as enhancers) plays an important role in the production of diverse morphology. However, a mechanistic understanding is often limited by the absence of methods for studying enhancers in species other than established model systems. Here, we sought to establish methods to identify and test enhancer activity in the red flour beetle, Tribolium castaneum To identify possible enhancer regions, we first obtained genome-wide chromatin profiles from various tissues and stages of Tribolium using FAIRE (formaldehyde-assisted isolation of regulatory elements)-sequencing. Comparison of these profiles revealed a distinct set of open chromatin regions in each tissue and at each stage. In addition, comparison of the FAIRE data with sets of co

SUBMITTER: Lai YT 

PROVIDER: S-EPMC11736658 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature