The empowering influence of air-liquid interface culture on skin organoid hair follicle development.

ABSTRACT:

Background

Rodent models have been widely used to investigate skin development, but do not account for significant differences in composition compared to human skin. On the other hand, two-dimensional and three-dimensional engineered skin models still lack the complex features of human skin such as appendages and pigmentation. Recently, hair follicle containing skin organoids (SKOs) with a stratified epidermis, and dermis layer have been generated as floating spheres from human-induced pluripotent stem cells (hiPSCs).

Methods

The current study aims to investigate the generation of hiPSCs-derived SKOs using an air-liquid interface (ALI) model on transwell membranes (T-SKOs) and compares their development with conventional floating culture in low-attachment plates (F-SKOs).

SUBMITTER: Sun J 

PROVIDER: S-EPMC11736897 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature