ARP2/3 complex affects myofibroblast differentiation and migration in pancreatic ductal adenocarcinoma.

ABSTRACT: The ARP2/3 complex, which orchestrates actin cytoskeleton organization and lamellipodia formation, has been implicated in the initiation of pancreatic ductal adenocarcinoma (PDAC). This study aims to clarify its impact on the activity of cancer-associated fibroblasts (CAFs), key players in PDAC progression, and patient outcomes. Early pancreatic carcinogenesis was modeled in p48^Cre; LSL-Kras^G12D mice with caerulein-induced pancreatitis, complemented by in vitro studies on human immortalized pancreatic stellate cells (PSCs) and primary PDAC-derived CAFs. Data were gained from microarray analysis, RNA sequencing (RNA-seq), and single-cell RNA sequencing (sc-RNA-seq), with subsequent bioinformatics analysis. We uncovered a specific transcriptional signature associated wi

SUBMITTER: Sun Y 

PROVIDER: S-EPMC11737003 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature