Transcriptome analysis of novel B16 melanoma metastatic variants generated by serial intracarotid artery injection.

ABSTRACT: The incidence of brain metastases (BrM) in patients with metastatic melanoma is reported to be 30-50% and constitutes the third most frequent BrM after breast and renal cancers. Treatment strategies including surgical resection, stereotactic radiation, and immunotherapy have improved clinical response rates and overall survival, but the changes that occur in circulating melanoma cells to promote invasion of the brain are not fully understood. To investigate brain tropism, we generated new variants of the B16 mouse melanoma model by serially passaging B16 cells through the brain of immune competent syngeneic C57BL/6 mice. Cells were injected into the right carotid artery and recovered from the brain after the mice had reached the study endpoint due to tumor burden, then expanded in vitro an

SUBMITTER: Kienzler JC 

PROVIDER: S-EPMC11737150 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature