Single-Cell RNA Sequencing before and after Light Chain Escape Reveals Intrapatient Multiple Myeloma Subpopulations with Divergent Osteolytic Gene Expression.

ABSTRACT:

Abstract

High-risk multiple myeloma is genomically unstable, comprising heterogeneous populations of tumor cells that evolve over time. Light chain escape (LCE) is a clinical phenomenon observed when light chains rise separately from M-spike values, which implies divergent tumor evolution. We sought to understand LCE by performing high-depth transcriptomic and phenotypic studies. We performed single-cell RNA-sequencing (scRNA-seq) and ex vivo drug sensitivity profiling on serial bone marrow biopsies from a patient with LCE at diagnosis, first relapse, and relapsed/refractory timepoints. scRNA-seq revealed distinct transcriptomic subpopulations with phenotypes that could be tracked separately by clinical serum light chain and M-spike values. Genes differentially expressed between su

SUBMITTER: Ohlstrom D 

PROVIDER: S-EPMC11737298 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature