Ion Mobility-Assisted Free Radical-Initiated Peptide Sequencing.

ABSTRACT: Free radical-initiated peptide sequencing (FRIPS) is a tandem mass spectrometry technique (MS/MS) that enables radical-based dissociation on instruments only capable of collisional activation. In FRIPS, peptides are chemically-derivatized with a compound that undergoes homolytic cleavage and generates radicals upon collisional activation. These radicals then propagate through the peptide backbone enabling the sequencing of peptide ions. This MS/MS technique has shown promise in sequencing post-translationally modified peptides, but it is typically performed in an MS³ workflow and single-step MS/MS approaches result in the generation of both collisional- and radical-driven dissociation products and highly complex spectra. Recently, our group developed a method to dissociate pepti

SUBMITTER: Osho KE 

PROVIDER: S-EPMC11737517 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature