Actin cable dynamics in budding yeast.

ABSTRACT: Actin cables, bundles of actin filaments that align along the long axis of budding yeast, are crucial for establishment of cell polarity. We fused green fluorescent protein (GFP) to actin binding protein 140 (Abp140p) and visualized actin cable dynamics in living yeast. We detected two populations of actin cables: (i) bud-associated cables, which extend from the bud along the mother-bud axis, and (ii) randomly oriented cables, which are relatively short. Time-lapse imaging of Abp140p-GFP revealed an apparent increase in the length of bud-associated actin cables. Analysis of movement of Abp140p-GFP fiduciary marks on bud-associated cables and fluorescence loss in photobleaching experiments revealed that this apparent elongation occurs by assembly of new material at the end of the cable with

SUBMITTER: Yang HC 

PROVIDER: S-EPMC117377 | biostudies-literature | 2002 Jan

REPOSITORIES: biostudies-literature