Target-agnostic identification of human antibodies to <i>Plasmodium falciparum</i> sexual forms reveals cross-stage recognition of glutamate-rich repeats.

ABSTRACT: Circulating sexual stages of Plasmodium falciparum (Pf) can be transmitted from humans to mosquitoes, thereby furthering the spread of malaria in the population. It is well established that antibodies can efficiently block parasite transmission. In search for naturally acquired antibodies targets on sexual stages, we established an efficient method for target-agnostic single B cell activation followed by high-throughput selection of human monoclonal antibodies (mAbs) reactive to sexual stages of Pf in the form of gametes and gametocyte extracts. We isolated mAbs reactive against a range of Pf proteins including well-established targets Pfs48/45 and Pfs230. One mAb, B1E11K, was cross-reactive to various proteins containing glutamate-rich repetitive elements expressed at

SUBMITTER: Amen A 

PROVIDER: S-EPMC11737873 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature