A Lambda-evo (λ_evo) phage platform for Zika virus E_DIII protein display.

ABSTRACT: One of the most significant bacteriophage technologies is phage display, in which heterologous peptides are exhibited on the virion surface. This work describes the display of λ decorative protein D_λ linked to the E protein domain III of Zika virus (D_λ-ZE_DIII), to the GFP protein (D_λ-GFP), or to different domain III epitopes of the E_ZIKV protein (D_λ-TD), exhibited on the surface of an in vitro evolved lambda phage (λ_evo). This phage harbors a gene D deletion and was subjected to directed evolution using Escherichia coli W3110/pD_λ-ZE_DIII as background. After 20 days (20 cycles of dilution), the λ_evo phage developed a ~ 22% genome deletion affecting the non-essential λ b region, rend

SUBMITTER: Negrete-Mendez H 

PROVIDER: S-EPMC11739229 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature