Structure and function of a β-1,2-galactosidase from Bacteroides xylanisolvens, an intestinal bacterium.

ABSTRACT: Galactosides are major carbohydrates that are found in plant cell walls and various prebiotic oligosaccharides. Studying the detailed biochemical functions of β-galactosidases in degrading these carbohydrates is important. In particular, identifying β-galactosidases with new substrate specificities could help in the production of potentially beneficial oligosaccharides. In this study, we identify a β-galactosidase with novel substrate specificity from Bacteroides xylanisolvens, an intestinal bacterium. The enzyme do not show hydrolytic activity toward natural β-galactosides during the first screening. However, when α-D-galactosyl fluoride (α-GalF) as a donor substrate and galactose or D-fucose as an acceptor substrate are incubated with a nucleophile mutant, reaction products are detected.

SUBMITTER: Nakazawa Y 

PROVIDER: S-EPMC11739564 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature