GPVI-mediated thrombus stabilization of shear-induced platelet aggregates in a microfluidic stenosis.

ABSTRACT: Supraphysiological shear rates (>2000 s^-1) amplify von Willebrand factor unfurling and increase platelet activation and adhesion. These elevated shear rates and shear rate gradients also play a role in shear-induced platelet aggregation (SIPA). The primary objective of this study is to investigate the contributions of major binding receptors to platelet deposition and SIPA in a stenotic model. Microfluidic channels with stenotic contractions ranging from 0% to 75% are fabricated and coated with human type I collagen at 100 μg/mL. Fresh human blood is reconstituted to 40% hematocrit and treated to stain platelets. Platelet receptors α_IIbβ₃, GPIb, or GPVI are blocked with inhibitory antibodies or proteins to reduce platelet function under flow at 500, 1000, 5

SUBMITTER: Watson CT 

PROVIDER: S-EPMC11739887 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature