Immobilized Nucleoside 2'-Deoxyribosyltransferases from Extremophiles for Nucleoside Biocatalysis.

ABSTRACT: The synthesis of nucleosides is crucial for pharmaceutical and biotechnological applications, acting as drugs and as essential building blocks for numerous therapeutic agents. However, most enzymes employed in nucleoside biocatalysis are not recycled, possess limited stability, and have strict substrate selection for ribonucleosides or 2'deoxyribonucleosides. We employed 2'-deoxyribonucleoside transferase (NDT) enzymes from thermophilic and psychrophilic bacteria to demonstrate they can be immobilized to enhance specific activity, stability, and recyclability. NDT enzymes from Chroococcidiopsis thermalis (CtNDT), and Bacillus psychrosaccharolyticus (BpNDT) were immobilized by covalent attachment to chitosan beads. A double mutant of CtNDT, capable of gene

SUBMITTER: Antonio Hernandez Martinez S 

PROVIDER: S-EPMC11740241 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature