Comparing the Extent of Methionine Oxidation in the Prion and Native Conformations of PrP.

ABSTRACT: Scrapie is a prion disease of sheep and goats. Prions (PrP^Sc) replicate by inducing a natively expressed protein (PrP^C) to refold into the prion conformation. PrP^C and PrP^Sc contain a disproportionately large number of methionines. Surface exposed methionines are more prone to chemical oxidation. Chemical oxidation is a means of measuring the surface exposure of the methionines in a prion, as these covalent changes are retained after an oxidized prion is denatured prior to analysis. Scrapie prions and recombinant sheep prion protein were oxidized in 0, 10, 20, or 50 mM solutions of hydrogen peroxide. The samples were digested with trypsin or trypsin followed by chymotrypsin to yield a set of peptides (TNMK, MLGSAMSR, ENMYR, IMER, VVEQMCITQYQR) co

SUBMITTER: Silva CJ 

PROVIDER: S-EPMC11740255 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature