Single-cell transcriptomic analysis reveals characteristic feature of macrophage reprogramming in liver Mallory-Denk bodies pathogenesis.

ABSTRACT: Chronic liver diseases are highly linked with mitochondrial dysfunction and macrophage infiltration. Mallory-Denk bodies (MDBs) are protein aggregates associated with hepatic inflammation, and MDBs pathogenesis could be induced in mice by feeding 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC). Here, we investigate the macrophage heterogeneity and the role of macrophage during MDBs pathogenesis on DDC-induced MDBs mouse model by single-nucleus RNA sequencing (snRNA-seq). We defined liver macrophages into four distinct subsets including monocyte-derived macrophages (MDMs) subset and three Kupffer cells (KCs) subsets (Gpnmb^high KCs, Peam1^high KCs, and Gpnmb^low Pecam1^low KCs). Particularly, we identified a novel Gpnmb^high KCs subset as li

SUBMITTER: Fang Z 

PROVIDER: S-EPMC11740356 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature