Interaction of STIL with FOXM1 regulates SF3A3 transcription in the hepatocellular carcinoma development.

ABSTRACT:

Background

Dysregulation of SF3A3 has been related to the development of many cancers. Here, we investigated the functional role of SF3A3 in hepatocellular carcinoma (HCC).

Methods

SF3A3 expression in HCC tissues and cell lines was examined using RT-qPCR. Changes in malignant behavior of HCC cells after downregulation of SF3A3 were assessed by EdU, colony formation, flow cytometry, wound healing, and Transwell invasion assays. Multiple datasets were combined to identify the upstream modifiers of SF3A3. The binding relationship between STIL and FOXM1 was explored by co-IP assay, and the effect of STIL and FOXM1 on the binding of FOXM1 at the SF3A3 promoter was detected by ChIP-qPCR assay. A xenograft tumor model was established to explore the changes of tumors in vivo, and th

SUBMITTER: Zhang H 

PROVIDER: S-EPMC11740530 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature