Useful experimental aspects of small-wedge synchrotron crystallography for accurate structure analysis of protein molecules.

ABSTRACT: Recent advances in low-emittance synchrotron X-ray technology and highly sensitive photon-counting detectors have revolutionized protein micro-crystallography in structural biology. These developments and improvements to sample-exchange robots and beamline control have paved the way for automated and efficient unattended data collection. This study analyzed protein crystal structures such as type 2 angiotensin II receptor, CNNM/CorC membrane proteins and polyhedral protein crystals using small-wedge synchrotron crystallography (SWSX), which dramatically improves measurement efficiency through automated measurement. We evaluated the data quality using SWSX, focusing on `massive data collection'. In this context, `massive' refers to data sets with a multiplicity exceeding 100. The findings c

SUBMITTER: Hirata K 

PROVIDER: S-EPMC11740584 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature