Benchmarking of a time-saving and scalable protocol for the extraction of DNA from diverse viromes.

ABSTRACT: The virome, composed of viruses inhabiting diverse ecosystems, significantly influences microbial community dynamics and host health. The phenol-chloroform DNA extraction protocol for viromes, though effective, is time-intensive and requires the use of multiple toxic chemicals. This study introduces a streamlined, scalable protocol for DNA extraction using a commercially-available kit as an alternative, assessing its performance against the phenol-chloroform method across human fecal, mouse fecal, and soil samples. No significant differences in virome diversity or community composition were seen between methods. Most viral operational taxonomic units (vOTUs) were common to both methods, with only a small percentage unique to either approach. Alpha- and beta-diversity analyses showed no sig

SUBMITTER: Shamash M 

PROVIDER: S-EPMC11740735 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature