Genome Combination Improvement Strategy Promotes Efficient Spinosyn Biosynthesis in <i>Saccharopolyspora spinosa</i>.

ABSTRACT: Spinosyns are secondary metabolites produced by Saccharopolyspora spinosa known for their potent insecticidal properties and broad pesticidal spectrum. We report significant advancements in spinosyn biosynthesis achieved through a genome combination improvement strategy in S. spinosa. By integrating modified genome shuffling with ultraviolet mutation and multiomics analysis, we developed a high-yield spinosyn strain designated as YX2. The levels of most proteins and metabolites linked to primary metabolism and spinosyn biosynthesis were greater in this strain than those in S. spinosa. Based on these insights, we overexpressed 15 relevant functional genes to enhance the conversion of fatty acids into acetyl-coenzyme A. Notably, the overexpression of acd (YX2_3432

SUBMITTER: Xia Z 

PROVIDER: S-EPMC11740894 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature