Bacterial and viral assemblages in ulcerative colitis patients following fecal microbiota and fecal filtrate transfer.

ABSTRACT: Fecal microbiota filtrate transfer is discussed as a safe alternative to fecal microbiota transfer (FMT) to treat ulcerative colitis. We investigated modulation of viral and bacterial composition during fecal microbiota filtrate transfer followed by FMT in six patients with active ulcerative colitis (where clinical activity improved in three patients after filtrate transfer) and combined 16S ribosomal RNA gene amplicon sequencing with a virome analysis pipeline including fast viral particle enrichment and metagenome mapping to detect frequencies of 45,033 reference bacteriophage genomes. We showed that after antibiotic treatment and during filtrate transfer, the bacterial community typically adopted a stable composition distinct to that before antibiotic treatment, with no change toward a

SUBMITTER: Junca H 

PROVIDER: S-EPMC11740987 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature