Stress Granule Induction in Rat Retinas Damaged by Constant LED Light.

ABSTRACT:

Purpose

Stress granules (SGs) are cytoplasmic biocondensates formed in response to various cellular stressors, contributing to cell survival. Although implicated in diverse pathologies, their role in retinal degeneration (RD) remains unclear. We aimed to investigate SG formation in the retina and its induction by excessive LED light in an RD model.

Methods

Rat retinas were immunohistochemically analyzed for SG markers G3BP1 and eIF3, and SGs were also visualized by RNA fluorescence in situ hybridization. Additionally, SGs were induced in primary retinal cell and eyeball cultures using sodium arsenite. Light exposure experiments used LED lamps with a color temperature of 5500 K and 200 lux intensity for short-term or two- to eight-day exposures.

Results

SGs were predom

SUBMITTER: Benedetto MM 

PROVIDER: S-EPMC11741064 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature