Molecular-Level Insights into Recalcitrant Ozonation Products from Effluent Organic Matter.

ABSTRACT: Wastewater ozonation is commonly employed to enhance the subsequent biodegradation of effluent organic matter (EfOM) and contaminants of concern. However, there is evidence suggesting the formation of recalcitrant ozonation products (OPs) from EfOM. To investigate the biodegradability of OPs we conducted batch biodegradation experiments using wastewater effluent ozonated with mass-labeled (¹⁸O) ozone. Molecular level analysis of EfOM was performed using reversed-phase liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry (LC-FT-ICR MS) after 3 and 28 days in batch bioreactors. The use of mass labeling allowed for the unambiguous detection of OPs, with 2933 labeled OP features identified in the ozonated EfOM. Furthermore, employing polarit

SUBMITTER: Jennings EK 

PROVIDER: S-EPMC11741107 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature