Flow-induced bending of flagella controls bacterial surface behavior.

ABSTRACT: Bacteria engage in surface-specific behaviors that are assumed to be driven by biological signaling. However, surface behaviors could be controlled by mechanical reorientation of bacterial appendages. Here, we use microfluidics and flagellar labeling to discover how shear force bends flagella to control surface behavior of the human pathogen Pseudomonas aeruginosa. By imaging flagellar rotation and using mutants with paralyzed flagella, we establish that flagellar rotation promotes surface departure in host-relevant shear regimes. Our single-cell experiments reveal two distinct subpopulations in flow: cells with their flagellum positioned upstream and cells with their flagellum positioned downstream. Shear force bends upstream flagella around the cell and blocks rotation. In contras

SUBMITTER: Palalay JS 

PROVIDER: S-EPMC11741401 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature