Monoclonal nanobodies alter the activity and assembly of the yeast vacuolar H⁺-ATPase.

ABSTRACT: The vacuolar ATPase (V-ATPase; V₁V_o) is a multi-subunit rotary nanomotor proton pump that acidifies organelles in virtually all eukaryotic cells, and extracellular spaces in some specialized tissues of higher organisms. Evidence suggests that metastatic breast cancers mislocalize V-ATPase to the plasma membrane to promote cell survival and facilitate metastasis, making the V-ATPase a potential drug target. We have generated a library of camelid single-domain antibodies (Nanobodies; Nbs) against lipid-nanodisc reconstituted yeast V-ATPase V_o proton channel subcomplex. Here, we present an in-depth characterization of three anti-V_o Nbs using biochemical and biophysical in vitro experiments. We find that the Nbs bind V_o with high af

SUBMITTER: Knight K 

PROVIDER: S-EPMC11741422 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature