Confirming Subarachnoid Hemorrhage Induction in the Endovascular Puncture Mouse Model.

ABSTRACT:

Background and purpose

Experimental studies of subarachnoid hemorrhage (SAH) using the endovascular perforation model routinely use either cerebral blood flow (CBF) or intracranial pressure (ICP) monitoring to confirm SAH induction. We designed this study to test the hypothesis that contralateral CBF is a better marker of SAH induction than ipsilateral CBF.

Methods

Sixty-eight adult C57BL/6J mice were subjected to endovascular perforation. Mice were monitored using laser Doppler (for relative CBF) or ICP probes.

Results

The ipsilateral CBF significantly decreased prior to ICP rising. However, reduction of the CBF in the contralateral hemisphere occurred at the same time as the rise in ICP. When CBF is monitored on both hemispheres simultaneously, the drop in ipsilater

SUBMITTER: Dienel A 

PROVIDER: S-EPMC11741539 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature