Design and Characterization of a Gene-Encoding DNA Nanoparticle in a Cell-Free Transcription-Translation System.

ABSTRACT: DNA nanotechnology has made initial progress toward developing gene-encoded DNA origami nanoparticles (NPs) that display potential utility for future gene therapy applications. However, due to the challenges involved with gene delivery into cells including transport through the membrane, intracellular targeting, and inherent expression of nucleases along with interference from other active proteins, it can be difficult to more directly study the effect of DNA NP design on subsequent gene expression. In this work, we demonstrate an approach for studying the expression of gene-encoding DNA origami NPs without the use of cells. We utilize a pure E. coli-derived cell-free transcription-translation (TXTL) system, which is composed of optimized components from bacterial expression, for be

SUBMITTER: Galvan AR 

PROVIDER: S-EPMC11741557 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature