Twist-free ultralight two-photon fiberscope enabling neuroimaging on freely rotating/walking mice.

ABSTRACT: Lightweight and head-mountable scanning nonlinear fiberscope technologies offer an exciting opportunity for enabling mechanistic exploration of ensemble neural activities with subcellular resolution on freely behaving rodents. The tether of the fiberscope, consisting of an optical fiber and scanner drive wires, however, restricts the mouse's movement and consequently precludes free rotation and limits the freedom of walking. Here we present the first twist-free two-photon fiberscope technology for enabling neuroimaging on freely rotating/walking mice. The technology equips a scanning fiberscope with active rotational tracking and compensation capabilities through an optoelectrical commutator (OEC) to allow the animal to rotate and walk in arbitrary patterns during two-photon fluorescence (

SUBMITTER: Li A 

PROVIDER: S-EPMC11741673 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature