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Evaluation of bacterial biofilm, smear layer, and debris removal efficacy of a hydro-dynamic cavitation system with physiological saline using a new ex vivo model: a CLSM and SEM study.


ABSTRACT:

Introduction

To evaluate the bacterial biofilm, smear layer and debris removal efficacy of a hydro-dynamic cavitation system with physiological saline using a new ex vivo model.

Methods

Seventy-five dentin discs were prepared from fifty-four extracted teeth. Seventy-five artificial root sockets were prepared. Sixty dentin discs were used to grow 3-week-old bacterial biofilms, while smear layer and debris were produced on fifteen dentin discs. These dentin discs were adhered to the middle third of the artificial root canals. The sixty ex vivo models with biofilm-covered dentin discs were divided into six groups: control, needle with physiological saline, ultrasonic with physiological saline, Odne™ Clean with physiological saline, needle with 3% NaOCl, and ultrasonic with 3% N

SUBMITTER: Liu H 

PROVIDER: S-EPMC11742200 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature