Enhancing abscisic acid production in <i>Botrytis cinerea</i> through metabolic engineering based on a constitutive promoter library.

ABSTRACT: Abscisic acid (ABA) is an important plant growth regulator with broad applications in agriculture, forestry, and other fields. Currently, the industrial production of ABA primarily relies on microbial fermentation using Botrytis cinerea, but its genetic toolbox is limited. To address this, we first screened 10 strong constitutive promoters from the genome of B. cinerea through transcriptomic analysis. The expression levels of the promoters covered a range of 3-4 orders of magnitude according to the measured β-glucuronidase activity. Subsequently, four promoters of different strength were used to balance the cofactor supply in B. cinerea. Overexpression of NADH kinase using the medium-strength promoter Pef1a significantly enhanced ABA production, resulting in a 3

SUBMITTER: Wang LR 

PROVIDER: S-EPMC11742572 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature