Microfluidic mixing probe: generating multiple concentration-varying flow dipoles.

ABSTRACT: This study advances microfluidic probe (MFP) technology through the development of a 3D-printed Microfluidic Mixing Probe (MMP), which integrates a built-in pre-mixer network of channels and features a lined array of paired injection and aspiration apertures. By combining the concepts of hydrodynamic flow confinements (HFCs) and "Christmas-tree" concentration gradient generation, the MMP can produce multiple concentration-varying flow dipoles, ranging from 0 to 100%, within an open microfluidic environment. This innovation overcomes previous limitations of MFPs, which only produced homogeneous bioreagents, by utilizing the pre-mixer to create distinct concentration of injected biochemicals. Experimental results with fluorescent dyes and the chemotherapeutic agent Cisplatin on MCF-7 cells c

SUBMITTER: Ali DS 

PROVIDER: S-EPMC11742695 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature