Improving protein hydrolysis and digestibility in Arthrospira platensis biomass through recombinant peptidases (EC 3.4): Opportunities for monogastric animal diets.

ABSTRACT: This study investigates the use of recombinant peptidases (EC 3.4) to improve protein hydrolysis and digestibility in Arthrospira platensis, with a focus on addressing the challenge of reduced protein bioavailability for monogastric animals due to resistant protein-pigment formations, such as phycocyanin, and increased digesta viscosity caused by jellification properties. A library of 192 peptidases was generated, from which 142 soluble peptidases were expressed in Escherichia coli and subsequently screened for activity against an A. platensis suspension in vitro. Among these peptidases, six promising candidates were identified for protein and peptide extraction from the microalga. These enzymes were tested individually, and in a mix (MIX6), and compared to comm

SUBMITTER: Spinola MP 

PROVIDER: S-EPMC11742843 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature